6VC2

LRH-1 bound to SS-RJW100 and a fragment of the Tif2 Coactivator

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	80
Detector technology	CCD
Collection date	2015-03-20
Detector	MAR CCD 130 mm
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	46.608, 46.608, 220.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.381 - 1.697
R-factor	0.2105
Rwork	0.210
R-free	0.22840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l11
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.760
High resolution limit [Å]	1.697	3.660	1.700
Rmerge	0.110	0.115	0.609
Rmeas	0.117	0.122	0.682
Rpim	0.039	0.041	0.292
Number of reflections	27544	3093	2314
<I/σ(I)>	14.9
Completeness [%]	97.4	98.5	84.9
Redundancy	6.7	8.2	3.7
CC(1/2)		0.991	0.788

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Sodium acetate, PEG 4000, glycerol