6V7E
Human Arginase1 Complexed with Bicyclic Inhibitor Compound 12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.720, 286.490, 67.120 |
| Unit cell angles | 90.00, 90.03, 90.00 |
Refinement procedure
| Resolution | 71.620 - 1.990 |
| R-factor | 0.216 |
| Rwork | 0.214 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6v7c |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.910 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | autoBUSTER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.620 | 2.020 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.110 | 0.640 |
| Number of reflections | 136926 | 6825 |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 98.5 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.990 | 0.750 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 10% MMT (pH 7.0) 0.1 M ammonium formate, 16-22% PEG 8000 |
