6V6Q

Crystal Structure of Monophosphorylated FGF Receptor 2 isoform IIIb with PTR657

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.11587
Spacegroup name	P 21 21 21
Unit cell lengths	63.970, 86.541, 254.162
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	82.060 - 2.460
R-factor	0.2129
Rwork	0.211
R-free	0.23880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2psq
RMSD bond length	0.007
RMSD bond angle	1.393
Data reduction software	MOSFLM
Data scaling software	Aimless (3.3.21)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	127.080	2.590
High resolution limit [Å]	2.460	2.460
Rmerge	0.098	1.088
Rmeas	0.112	1.232
Rpim	0.041	0.564
Total number of observations	338930	21237
Number of reflections	50447	5926
<I/σ(I)>	12.7	1.8
Completeness [%]	96.6	80.3
Redundancy	6.7	3.6
CC(1/2)	0.998	0.326

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.6	293	160mM TMAO, 20% PEG 2000, 100mM Tris pH 8.6