6V4R
Crystal structure of a chimeric MR78-like antibody chimera-1 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-11-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 133.780, 133.780, 91.910 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 57.930 - 3.480 |
| R-factor | 0.2961 |
| Rwork | 0.295 |
| R-free | 0.32730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jrp |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.178 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.930 | 3.540 |
| High resolution limit [Å] | 3.480 | 3.480 |
| Rmeas | 0.342 | 4.159 |
| Rpim | 0.084 | 0.995 |
| Total number of observations | 206074 | |
| Number of reflections | 12393 | 618 |
| <I/σ(I)> | 8.8 | 1.1 |
| Completeness [%] | 99.2 | 98.7 |
| Redundancy | 16.6 | 17.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 287 | 4.5 M NaCl, 0.1 M HEPES, pH 7.5 |
