6UXJ

Structure of serine hydroxymethyltransferase 8 from Glycine max cultivar Essex complexed with PLP-glycine and 5-formyltetrahydrofolate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2018-04-29
Detector	RDI CMOS_8M
Wavelength(s)	1.00
Spacegroup name	P 1 21 1
Unit cell lengths	86.889, 90.764, 146.850
Unit cell angles	90.00, 90.81, 90.00

Refinement procedure

Resolution	45.382 - 1.400
R-factor	0.1659
Rwork	0.165
R-free	0.19370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6uxh
Data reduction software	XDS
Data scaling software	Aimless (0.6.3)
Phasing software	PHASER
Refinement software	PHENIX (1.14-3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.950	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.070	1.019
Rmeas	0.083	1.225
Rpim	0.044	0.670
Total number of observations	1533812	68936
Number of reflections	440251	21837
<I/σ(I)>	10.2	1
Completeness [%]	98.5	98.9
Redundancy	3.5	3.2
CC(1/2)	0.998	0.504

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.24 M trimethylamine N-oxide, 0.1 M Tris (pH 8.5), 20% (w/v) PEG MME 2000, 10 mM 5-formyl tetrahydrofolate, 20 mM glycine