6UX9
Crystal Structure Analysis of PIP4K2A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.230, 88.580, 105.780 |
| Unit cell angles | 90.00, 92.91, 90.00 |
Refinement procedure
| Resolution | 45.368 - 1.710 |
| R-factor | 0.1784 |
| Rwork | 0.177 |
| R-free | 0.20360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6osp |
| Data reduction software | xia2 |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.370 | 45.380 | 1.740 |
| High resolution limit [Å] | 1.710 | 4.640 | 1.710 |
| Rmeas | 0.048 | 0.033 | 1.336 |
| Rpim | 0.026 | 0.018 | 0.703 |
| Total number of observations | 291606 | 14593 | 14742 |
| Number of reflections | 86013 | 4334 | 4252 |
| <I/σ(I)> | 12.4 | 32 | 1.2 |
| Completeness [%] | 97.8 | 96 | 97.7 |
| Redundancy | 3.4 | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M sodium citrate, pH 6.5 12% PEG 5000 MME |
