6UX9

Crystal Structure Analysis of PIP4K2A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-15
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	44.230, 88.580, 105.780
Unit cell angles	90.00, 92.91, 90.00

Refinement procedure

Resolution	45.368 - 1.710
R-factor	0.1784
Rwork	0.177
R-free	0.20360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6osp
Data reduction software	xia2
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.370	45.380	1.740
High resolution limit [Å]	1.710	4.640	1.710
Rmeas	0.048	0.033	1.336
Rpim	0.026	0.018	0.703
Total number of observations	291606	14593	14742
Number of reflections	86013	4334	4252
<I/σ(I)>	12.4	32	1.2
Completeness [%]	97.8	96	97.7
Redundancy	3.4	3.4	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M sodium citrate, pH 6.5 12% PEG 5000 MME