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6UX9

Crystal Structure Analysis of PIP4K2A

Summary for 6UX9
Entry DOI10.2210/pdb6ux9/pdb
DescriptorPhosphatidylinositol 5-phosphate 4-kinase type-2 alpha, N-[4-(5-{(Z)-[(2E)-2-imino-4-oxo-1,3-thiazolidin-5-ylidene]methyl}pyridin-3-yl)phenyl]methanesulfonamide (3 entities in total)
Functional Keywordskinase, signaling protein, transferase
Biological sourceHomo sapiens (Human)
Total number of polymer chains2
Total formula weight86490.21
Authors
Seo, H.-S.,Dhe-Paganon, S. (deposition date: 2019-11-07, release date: 2020-12-09, Last modification date: 2023-10-11)
Primary citationManz, T.D.,Sivakumaren, S.C.,Ferguson, F.M.,Zhang, T.,Yasgar, A.,Seo, H.S.,Ficarro, S.B.,Card, J.D.,Shim, H.,Miduturu, C.V.,Simeonov, A.,Shen, M.,Marto, J.A.,Dhe-Paganon, S.,Hall, M.D.,Cantley, L.C.,Gray, N.S.
Discovery and Structure-Activity Relationship Study of ( Z )-5-Methylenethiazolidin-4-one Derivatives as Potent and Selective Pan-phosphatidylinositol 5-Phosphate 4-Kinase Inhibitors.
J.Med.Chem., 63:4880-4895, 2020
Cited by
PubMed Abstract: Due to their role in many important signaling pathways, phosphatidylinositol 5-phosphate 4-kinases (PI5P4Ks) are attractive targets for the development of experimental therapeutics for cancer, metabolic, and immunological disorders. Recent efforts to develop small molecule inhibitors for these lipid kinases resulted in compounds with low- to sub-micromolar potencies. Here, we report the identification of CVM-05-002 using a high-throughput screen of PI5P4Kα against our in-house kinase inhibitor library. CVM-05-002 is a potent and selective inhibitor of PI5P4Ks, and a 1.7 Å X-ray structure reveals its binding interactions in the ATP-binding pocket. Further investigation of the structure-activity relationship led to the development of compound , replacing the rhodanine-like moiety present in CVM-05-002 with an indole, a potent pan-PI5P4K inhibitor with excellent kinome-wide selectivity. Finally, we employed isothermal cellular thermal shift assays (CETSAs) to demonstrate the effective cellular target engagement of PI5P4Kα and -β by the inhibitors in HEK 293T cells.
PubMed: 32298120
DOI: 10.1021/acs.jmedchem.0c00227
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.71 Å)
Structure validation

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