6UUQ

Structure of Calcineurin bound to RCAN1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-27
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.03320
Spacegroup name	P 21 21 21
Unit cell lengths	57.841, 71.159, 92.049
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.014 - 1.849
R-factor	0.1655
Rwork	0.164
R-free	0.20490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f0z
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.880
High resolution limit [Å]	1.849	1.849
Rmeas	0.091	0.735
Number of reflections	33218	1644
<I/σ(I)>	24.5
Completeness [%]	99.9
Redundancy	4.9
CC(1/2)		0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	0.1 M Sodium cacodylate, 12% Peg 8000, 0.1 M Calcium acetate