6URQ

Complex structure of human poly-ADP-ribosyltransferase TNKS1 ARC2-ARC3 and P antigen family member 4 (PAGE4)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	134.908, 101.035, 75.418
Unit cell angles	90.00, 107.23, 90.00

Refinement procedure

Resolution	39.750 - 2.050
R-factor	0.2062
Rwork	0.204
R-free	0.24630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3utm
RMSD bond length	0.007
RMSD bond angle	0.822
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER ((1.14_3260: ???))
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.750	2.100
High resolution limit [Å]	2.050	2.050
Rmeas	0.085	0.834
Rpim	0.043	0.422
Number of reflections	60143	4432
<I/σ(I)>	10.2	2.1
Completeness [%]	99.4	98.7
Redundancy	3.8	3.8
CC(1/2)	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	298	100 mM Ammonium Sulfate, 10 mM DTT, 100 mM MES pH 6.0, 7% w/v PEG4000, 1% 1,6-Hexanediol