6URH

Crystal structure of broadly neutralizing antibody AR3X in complex with Hepatitis C virus envelope glycoprotein E2 ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	64.730, 69.231, 176.699
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.493 - 2.200
R-factor	0.1887
Rwork	0.186
R-free	0.23250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mwf 6mei
Data reduction software	MOSFLM (7.2.2)
Data scaling software	Aimless (0.7.2)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.730	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.112	1.400
Rmeas	0.123	1.537
Rpim	0.050	0.626
Total number of observations	243765	20579
Number of reflections	40956	3516
<I/σ(I)>	9	1.4
Completeness [%]	99.4	99.6
Redundancy	6	5.9
CC(1/2)	0.993	0.375

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.25 M ammonium tartrate dibasic pH 7.0, 20% PEG 3350