6UQC

Mouse IgG2a Bispecific Fc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000000
Spacegroup name	P 1
Unit cell lengths	64.270, 64.400, 88.980
Unit cell angles	76.19, 72.17, 61.48

Refinement procedure

Resolution	35.650 - 1.870
R-factor	0.1956
Rwork	0.194
R-free	0.22870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1igt 5vaa
RMSD bond length	0.007
RMSD bond angle	0.988
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.650	1.939
High resolution limit [Å]	1.870	1.872
Rmerge	0.031	0.282
Rmeas	0.044	0.399
Rpim	0.031	0.282
Number of reflections	82860	6078
<I/σ(I)>	13.61	2.49
Completeness [%]	84.7
Redundancy	1.8
CC(1/2)	0.998	0.873

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.9	295.15	21% PEG10000 and 0.1M bicine pH 7.9