6UPX
RNA polymerase II elongation complex with 5-guanidinohydantoin lesion in state 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-09-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00003 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 168.988, 223.314, 193.102 |
| Unit cell angles | 90.00, 101.03, 90.00 |
Refinement procedure
| Resolution | 49.295 - 3.400 |
| R-factor | 0.2206 |
| Rwork | 0.220 |
| R-free | 0.26620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e2j |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.2) |
| Phasing software | PHASER (2.8.1) |
| Refinement software | PHENIX (1.13) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.300 | 3.360 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.428 | 3.818 |
| Rmeas | 0.459 | 4.092 |
| Rpim | 0.165 | 1.468 |
| Total number of observations | 811001 | 40721 |
| Number of reflections | 105382 | 5266 |
| <I/σ(I)> | 6.7 | 0.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.7 | 7.7 |
| CC(1/2) | 0.984 | 0.245 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000 |
