6UPR

Crystal Structure of GTPase Domain of Human Septin 2 / Septin 8 Heterocomplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-06
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97950
Spacegroup name	P 1 21 1
Unit cell lengths	52.754, 82.773, 78.144
Unit cell angles	90.00, 103.63, 90.00

Refinement procedure

Resolution	55.959 - 2.299
R-factor	0.2105
Rwork	0.208
R-free	0.25470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6upq
Data reduction software	xia2
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	82.800	82.800	2.360
High resolution limit [Å]	2.299	10.290	2.300
Rmerge	0.131	0.047	1.688
Rmeas	0.142	0.052	1.821
Rpim	0.054	0.021	0.680
Total number of observations		2161	14951
Number of reflections	28853	348	2108
<I/σ(I)>	8	34.6	1
Completeness [%]	99.2	99.5	99.5
Redundancy	6.9	6.2	7.1
CC(1/2)	0.992	0.994	0.782

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	100mM MES/imidazole pH 6.5, 10% PEG 8000, 20% ethylene glycol and 20mM of each 1,6-hexanediol, 1-butanol, (RS)-1,2-propanediol, 2-propanol, 1,4-butanediol and 1,3-propanediol