6UML
Structural Basis for Thalidomide Teratogenicity Revealed by the Cereblon-DDB1-SALL4-Pomalidomide Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-06 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97931 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 77.409, 127.725, 110.174 |
Unit cell angles | 90.00, 109.69, 90.00 |
Refinement procedure
Resolution | 38.700 - 3.580 |
R-factor | 0.2066 |
Rwork | 0.204 |
R-free | 0.26710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tz4 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.679 |
Data reduction software | xia2 |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.700 | 3.640 |
High resolution limit [Å] | 3.580 | 3.580 |
Rmerge | 0.164 | 0.570 |
Rmeas | 0.195 | 0.673 |
Rpim | 0.104 | 0.355 |
Number of reflections | 23629 | 2336 |
<I/σ(I)> | 5.04 | |
Completeness [%] | 98.4 | 98.11 |
Redundancy | 3.4 | 3.5 |
CC(1/2) | 0.964 | 0.734 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 10% PEG MME 500, 8% PEG 20K, 210mM calcium acetate, 100mM tris pH 7.5 |