6UE6

PWWP1 domain of NSD2 in complex with MR837

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	69.210, 70.320, 228.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.300 - 2.400
R-factor	0.236
Rwork	0.235
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vc8
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.330	49.330	2.490
High resolution limit [Å]	2.400	8.980	2.400
Rmerge	0.084	0.043	0.997
Rmeas	0.092	0.048	1.084
Number of reflections	44525	974	4500
<I/σ(I)>	14	36.9	1.9
Completeness [%]	99.7
Redundancy	6.4	5.2	6.5
CC(1/2)	0.998	0.997	0.729

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	20% PEG3350, 0.2 M potassium thiocyanate