6U5L

Structure of human ULK4 in complex with an inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-23
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0331
Spacegroup name	P 65 2 2
Unit cell lengths	90.421, 90.421, 188.956
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.150 - 1.750
R-factor	0.1922
Rwork	0.191
R-free	0.21650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wnp
RMSD bond length	0.003
RMSD bond angle	0.722
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.5.12)
Phasing software	PHASER (2.5.7)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.150	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.157	3.065
Rmeas	0.163	3.173
Rpim	0.043	0.812
Number of reflections	46829	2541
<I/σ(I)>	11.7
Completeness [%]	99.8	100
Redundancy	15	15.5
CC(1/2)	0.999	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.25	298	PEG 6000, Imidazole