6U39

2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-27
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 2
Unit cell lengths	121.795, 161.656, 96.277
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.041 - 2.400
R-factor	0.2344
Rwork	0.232
R-free	0.28270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3g43
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.041	49.041	2.440
High resolution limit [Å]	2.300	6.840	2.300
Number of reflections	159034	6041	25711
<I/σ(I)>	17.06
Completeness [%]	97.5
Redundancy	2.93
CC(1/2)	0.999	0.998	0.799

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	ammonium sulfate