6TYY

Hedgehog autoprocessing mutant D46H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.1271
Spacegroup name	P 61
Unit cell lengths	78.381, 78.381, 37.351
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.656 - 1.360
R-factor	0.1722
Rwork	0.171
R-free	0.19490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1at0
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	1.380
High resolution limit [Å]	1.360	3.690	1.360
Rmerge	0.058	0.029	0.924
Rmeas	0.066	0.033	1.134
Rpim	0.030	0.015	0.642
Total number of observations	131992
Number of reflections	28219	1472	1367
<I/σ(I)>	11
Completeness [%]	99.6	98.8	96.3
Redundancy	4.7	4.6	2.9
CC(1/2)		0.999	0.624

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1 M Hepes, pH 7.5, 3.4 M sodium formate