6TYL

Crystal structure of mammalian Ric-8A:Galpha(i):nanobody complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	93.048, 144.726, 114.420
Unit cell angles	90.00, 94.66, 90.00

Refinement procedure

Resolution	39.530 - 3.300
Rwork	0.248
R-free	0.28700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6nmg
RMSD bond length	0.002
RMSD bond angle	0.548
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.580	3.900
High resolution limit [Å]	3.300	3.300
Rmeas	0.210	0.700
Rpim	0.110	0.380
Number of reflections	20707	1035
<I/σ(I)>	3.7	1.7
Completeness [%]	90.1
Redundancy	3.6
CC(1/2)	0.990	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		285	1.2-1.4M Sodium Malonate