6TUO
Crystal structure of Archaeoglobus fulgidus Argonaute protein with cognate DNA oligoduplex 5'-pATTGTACGTACAAT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97970 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 52.011, 99.634, 109.802 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.901 - 1.800 |
R-factor | 0.1883 |
Rwork | 0.184 |
R-free | 0.22690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pdbid 1ytu |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.2) |
Phasing software | MOLREP (11.6.04; 25.05.2018) |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 99.630 | 99.630 | 1.840 |
High resolution limit [Å] | 1.800 | 9.000 | 1.800 |
Rmerge | 0.044 | 0.027 | 0.815 |
Rmeas | 0.048 | 0.030 | 0.883 |
Rpim | 0.019 | 0.013 | 0.337 |
Total number of observations | 2894 | 21005 | |
Number of reflections | 53664 | 514 | 3128 |
<I/σ(I)> | 20.8 | 60.9 | 2.6 |
Completeness [%] | 99.9 | 99 | 100 |
Redundancy | 6.5 | 5.6 | 6.7 |
CC(1/2) | 0.999 | 0.998 | 0.900 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 280 | Na-Cacodylate pH 5.5 0.05 M, KCl_0.2 M, MgCl2 0.01 M, PEG4000 5% (w/v), glycerol 5% (v/v) |