6TC0

Crystal structure of MMS19-CIAO1-CIAO2B CIA targeting complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-06
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	107.400, 140.700, 327.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.600
R-factor	0.2591
Rwork	0.258
R-free	0.27710
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.312
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.730
High resolution limit [Å]	3.600	3.600
Number of reflections	111722	11172
<I/σ(I)>	12
Completeness [%]	98.4
Redundancy	41.4
CC(1/2)	1.000	0.124

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM HEPES pH 7.5, 25% PEG 3350