6T5T
Crystal structure of Archaeoglobus fulgidus Argonaute protein with cognate DNA oligoduplex 5'-pATTGTGGCCACAAT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97970 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 52.098, 99.551, 109.901 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.951 - 1.700 |
Rwork | 0.180 |
R-free | 0.21340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pdbid 1ytu |
Data reduction software | XDS (VERSION Jan 26, 2018 BUILT=20180126) |
Data scaling software | SCALA (3.3.22) |
Phasing software | MOLREP (Vers 11.6.04; 25.05.2018) |
Refinement software | PHENIX (1.13) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 99.551 | 99.551 | 1.790 |
High resolution limit [Å] | 1.700 | 5.380 | 1.700 |
Rmerge | 0.047 | 0.969 | |
Rmeas | 0.068 | 0.052 | 1.075 |
Rpim | 0.021 | 0.016 | 0.335 |
Total number of observations | 657679 | 20531 | 93225 |
Number of reflections | 63695 | 2222 | 9192 |
<I/σ(I)> | 20.8 | 61.1 | 2.3 |
Completeness [%] | 100.0 | 99.8 | 100 |
Redundancy | 10.3 | 9.2 | 10.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 280 | Na-Cacodylate pH 5.5 0.05 M, KCl_0.2 M, MgCl2 0.01 M, PEG4000 5% (w/v), glycerol 5% (v/v) |