6SZW
Asymmetric complex of Factor XII and kininogen with gC1qR/C1QBP/P32 is governed by allostery
Experimental procedure
| Experimental method | LAUE |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.69 - 2.07 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 106.290, 71.560, 115.857 |
| Unit cell angles | 90.00, 110.64, 90.00 |
Refinement procedure
| Resolution | 90.990 - 3.140 |
| R-factor | 0.1981 |
| Rwork | 0.195 |
| R-free | 0.25050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p32 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.463 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.990 | 3.360 |
| High resolution limit [Å] | 3.140 | 3.140 |
| Rmerge | 0.109 | |
| Number of reflections | 14379 | 2571 |
| <I/σ(I)> | 6.4 | |
| Completeness [%] | 99.8 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.953 | 0.793 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 283.15 | 0.1 M sodium cacodylate, 0.1 M calcium acetate, 12% (w/v) PEG 8000 |
