6SS4

Structure of arginase-2 in complex with the inhibitory human antigen-binding fragment Fab C0021181

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9184
Spacegroup name	P 3 2 1
Unit cell lengths	150.380, 150.380, 110.740
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.980 - 2.900
Rwork	0.266
R-free	0.31810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hze 6ss0
RMSD bond length	0.013
RMSD bond angle	2.012
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.980	3.060
High resolution limit [Å]	2.900	2.900
Rmerge	0.350	5.190
Number of reflections	32384	4672
<I/σ(I)>	11	0.7
Completeness [%]	100.0	100
Redundancy	20.2	20.8
CC(1/2)	1.000	0.320

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	291	100 mM MMT pH 5.0 (malic acid, MES, tris) 20% glycerol 10% PEG4000 15 mM NaNO3 15 mM Na2HPO4 15 mM (NH4)2SO4