6SS2

Structure of arginase-2 in complex with the inhibitory human antigen-binding fragment Fab C0021158

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9184
Spacegroup name	H 3
Unit cell lengths	149.061, 149.061, 123.287
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.368 - 2.400
Rwork	0.254
R-free	0.28420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ss4 6srx
RMSD bond length	0.005
RMSD bond angle	1.413
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.370	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.170	1.720
Number of reflections	39908	4198
<I/σ(I)>	7	1
Completeness [%]	99.9	100
Redundancy	5.2	5.4
CC(1/2)	0.990	0.360

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	Crystallisation condition: 2 M (NH4)2SO4 Seed condition (approx. final conc.) 20 mM MIB (malonate, imidazole, boric acid) 4% glycerol 2% PEG4000 9 mM NaNO3 9 mM Na2HPO4 9 mM (NH4)2SO4