6SQP

Crystal structure of Cat MDM2-S429E RING domain homodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.916
Spacegroup name	P 1 21 1
Unit cell lengths	29.240, 39.760, 104.380
Unit cell angles	90.00, 93.40, 90.00

Refinement procedure

Resolution	23.530 - 1.210
Rwork	0.171
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mnj
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2142: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.530	1.240
High resolution limit [Å]	1.210	1.210
Number of reflections	71796	5274
<I/σ(I)>	5.1
Completeness [%]	98.3
Redundancy	3.2
CC(1/2)	0.991	0.608

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		292	0.1 M MMT, pH 9.0 and 25% (w/v) PEG1500