6SQP

Crystal structure of Cat MDM2-S429E RING domain homodimer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	E3 ubiquitin-protein ligase Mdm2	polymer	63	7152.7	1	UniProt (Q7YRZ8) Pfam (PF13920)	Felis catus (Cat)	Double minute 2 protein,RING-type E3 ubiquitin transferase Mdm2,p53-binding protein Mdm2
2	B, D (B, D)	E3 ubiquitin-protein ligase Mdm2	polymer	68	7720.3	2	UniProt (Q7YRZ8) Pfam (PF13920)	Felis catus (Cat)	Double minute 2 protein,RING-type E3 ubiquitin transferase Mdm2,p53-binding protein Mdm2
3	C (C)	E3 ubiquitin-protein ligase Mdm2	polymer	65	7378.9	1	UniProt (Q7YRZ8) Pfam (PF13920)	Felis catus (Cat)	Double minute 2 protein,RING-type E3 ubiquitin transferase Mdm2,p53-binding protein Mdm2
4	E, F, H, I, J... (A, B, C, D)	ZINC ION	non-polymer		65.4	8	Chemie (ZN)
5	G, L (A, C)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
6	O (D)	NITRATE ION	non-polymer		62.0	1	Chemie (NO3)
7	P, Q, R, S (A, B, C, D)	water	water		18.0	243	Chemie (HOH)

Sequence modifications

A: 430 - 491 (UniProt: Q7YRZ8)

PDB	External Database	Details
Glu 429	-	engineered mutation
Thr 443	Gly 443	engineered mutation

B, D: 424 - 491 (UniProt: Q7YRZ8)

PDB	External Database	Details
Glu 429	Ser 429	engineered mutation
Thr 443	Gly 443	engineered mutation

C: 427 - 491 (UniProt: Q7YRZ8)

PDB	External Database	Details
Glu 429	Ser 429	engineered mutation
Thr 443	Gly 443	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	29972.3
Non-Polymers*	Number of molecules	11
	Total formula weight	656.2
All*	Total formula weight	30628.5

*Water molecules are not included.