6SQJ

Crystal structure of glycoprotein D of Equine Herpesvirus Type 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	71.860, 94.464, 101.296
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.803 - 2.245
R-factor	0.206
Rwork	0.203
R-free	0.25740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2c36
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.240	2.240
Number of reflections	33402	5140
<I/σ(I)>	11.71
Completeness [%]	99.1
Redundancy	6.5
CC(1/2)	0.998	0.586

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291.15	0.1 M Tris buffer, 0.2 M magnesium chloride, 30% (w/v) PEG 4000