6SN1

Crystal structure of the human INTS13-INTS14 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-11
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0000
Spacegroup name	P 21 21 2
Unit cell lengths	190.987, 115.354, 147.819
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.470 - 2.540
R-factor	0.2252
Rwork	0.224
R-free	0.24370
Structure solution method	MAD
RMSD bond length	0.002
RMSD bond angle	0.481
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MLPHARE
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.470	2.630
High resolution limit [Å]	2.540	2.540
Rmeas	0.149	3.806
Rpim	0.040	1.012
Number of reflections	107306	10162
<I/σ(I)>	16.2	0.6
Completeness [%]	99.5	95.33
Redundancy	13.7
CC(1/2)	0.999	0.354

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	0.1 M Bis-Tris (pH 6.0), 0.75 M AmSO4 and 1% (w/v) PEG 3350