6SLM

Crystal structure of full-length HPV31 E6 oncoprotein in complex with LXXLL peptide of ubiquitin ligase E6AP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-04
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 61 2 2
Unit cell lengths	113.640, 113.640, 185.970
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.210 - 2.800
R-factor	0.2319
Rwork	0.230
R-free	0.26820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xr8
RMSD bond length	0.003
RMSD bond angle	0.619
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.250	2.870
High resolution limit [Å]	2.800	2.800
Rmeas	0.230	2.076
Number of reflections	18049	1295
<I/σ(I)>	14.49	1.57
Completeness [%]	99.1
Redundancy	19.4	18.9
CC(1/2)	0.998	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	200mM tri-lithium citrate, 20% PEG 33350 protein concentration: 30mg/ml cryo condition: 35% glycerol