6SH2
Crystal structure of human neprilysin E584D in complex with C-type natriuretic peptide.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-13 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 109.267, 109.267, 112.579 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 72.440 - 2.600 |
| Rwork | 0.243 |
| R-free | 0.28660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gid |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.148 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.440 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 24386 | 2945 |
| <I/σ(I)> | 17.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 39.9 | |
| CC(1/2) | 1.000 | 0.604 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291.15 | Potassium nitrate, Sodium iodide, PEG 3350 |
