6S8S
Extended structure of the human DDX6 C-terminal domain in complex with an EDC3 FDF peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2008-06-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 172.425, 47.878, 65.757 |
| Unit cell angles | 90.00, 96.34, 90.00 |
Refinement procedure
| Resolution | 25.310 - 2.210 |
| R-factor | 0.181 |
| Rwork | 0.179 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wax |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.310 | 2.250 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Number of reflections | 25958 | 961 |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 95.6 | |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.1 M CAPS pH 10.5, 1.2 M NaH2PO4, 0.8 M K2HPO4, 0.2 M Li2SO4 |
