6RZ7

Crystal structure of the human cysteinyl leukotriene receptor 2 in complex with ONO-2570366 (F222 space group)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.07228
Spacegroup name	F 2 2 2
Unit cell lengths	81.830, 142.070, 171.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.930 - 2.430
R-factor	0.2309
Rwork	0.229
R-free	0.25870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rz4
RMSD bond length	0.007
RMSD bond angle	1.110
Data reduction software	XDS (20161205)
Data scaling software	XSCALE (20180319)
Phasing software	PHASER (1.12-2829)
Refinement software	PHENIX (1.12-2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.520
High resolution limit [Å]	2.430	2.430
Rmerge	0.194	2.168
Rpim	0.108	1.192
Number of reflections	17665
<I/σ(I)>	4.8	0.7
Completeness [%]	92.4	89.2
Redundancy	3.8	3.9
CC(1/2)	0.990	0.220

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	7	293	30 mM NH4 tartrate dibasic 24% PEG400 100 mM HEPES 7.0