6RPZ

Cytokine receptor-like factor 3 C-terminus residues 174-442: native collected with 1.7A wavelength

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.7007
Spacegroup name	C 1 2 1
Unit cell lengths	87.800, 40.600, 76.660
Unit cell angles	90.00, 99.80, 90.00

Refinement procedure

Resolution	32.150 - 1.740
R-factor	0.175
Rwork	0.173
R-free	0.20540
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	6rpx
RMSD bond length	0.011
RMSD bond angle	1.080
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.150	1.790
High resolution limit [Å]	1.740	1.740
Rmerge	0.057	0.778
Rmeas	0.064	0.913
Number of reflections	24310	6424
<I/σ(I)>	20	2.1
Completeness [%]	88.4	49.2
Redundancy	9.3	6.4
CC(1/2)	0.999	0.492

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	12 % PEG 3,350, 0.1M sodium acetate . Cryoprotected in 25 % ethylene glycol