6RLC
Crystal structure of the PDZ tandem of syntenin in complex with fragment F13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 |
| Unit cell lengths | 29.230, 57.512, 101.094 |
| Unit cell angles | 93.99, 96.08, 107.70 |
Refinement procedure
| Resolution | 50.020 - 2.200 |
| R-factor | 0.26105 |
| Rwork | 0.259 |
| R-free | 0.30141 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.652 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.020 | 2.279 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.069 | 0.368 |
| Rmeas | 0.082 | 0.435 |
| Rpim | 0.044 | 0.229 |
| Number of reflections | 30520 | 3035 |
| <I/σ(I)> | 9.74 | 3.02 |
| Completeness [%] | 96.8 | 95.92 |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 0.997 | 0.925 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | 100 mM Sodium Acetate pH 4.6, 200mM Ammonium Acetate, 22% PEG 3350 |
