6RIR

Crystal structure of phosphorylated Rab8a in complex with the Rab-binding domain of RILPL2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-31
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	60.333, 71.509, 114.784
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.410 - 1.767
R-factor	0.1805
Rwork	0.179
R-free	0.21050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lhw
RMSD bond length	0.007
RMSD bond angle	0.912
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.410	1.830
High resolution limit [Å]	1.767	1.767
Rmerge	0.069	0.949
Rmeas	0.075	1.036
Rpim	0.029	0.410
Number of reflections	49201	4719
<I/σ(I)>	16.4	1.69
Completeness [%]	99.5	96.33
Redundancy	6.6	6.2
CC(1/2)	0.999	0.649

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	290	8% PEG 8K 100mM Na HEPES