6RFU

In cellulo crystallization of Trypanosoma brucei IMP dehydrogenase enables the identification of ATP and GMP as genuine co-factors

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SLAC LCLS BEAMLINE CXI
Synchrotron site	SLAC LCLS
Beamline	CXI
Temperature [K]	291
Detector technology	PIXEL
Collection date	2012-01-28
Detector	CS-PAD XPP
Wavelength(s)	2.07
Spacegroup name	P 4 21 2
Unit cell lengths	209.000, 209.000, 92.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.300 - 2.800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jcn
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.300	2.870
High resolution limit [Å]	2.800	2.800
Number of reflections	50656	3557
<I/σ(I)>	4.14
Completeness [%]	100.0
Redundancy	187

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	IN CELL		291	In cellulo upon cell growth