6RFO
ERK2 MAP kinase with the activation loop of p38alpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-05 |
| Detector | DECTRIS PILATUS3 R CdTe 300K |
| Wavelength(s) | 0.972 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.999, 68.688, 60.231 |
| Unit cell angles | 90.00, 109.13, 90.00 |
Refinement procedure
| Resolution | 45.390 - 1.700 |
| R-factor | 0.15969 |
| Rwork | 0.156 |
| R-free | 0.21874 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4s31 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.894 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.390 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 40460 | 2160 |
| <I/σ(I)> | 10 | |
| Completeness [%] | 99.4 | |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.8 M ammonium sulfate, 0.1 M Bis-tris pH 6.8 or 6.5, 2% (w/v) PEG 550 |
