6RCT
Crystal structure of CLK3 in complex with T3-CLK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.91840 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.788, 123.329, 69.589 |
| Unit cell angles | 90.00, 92.64, 90.00 |
Refinement procedure
| Resolution | 69.520 - 2.320 |
| R-factor | 0.2079 |
| Rwork | 0.206 |
| R-free | 0.24330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2eu9 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.335 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 69.520 | 69.520 | 2.450 |
| High resolution limit [Å] | 2.320 | 7.340 | 2.320 |
| Rmerge | 0.136 | 0.073 | 0.763 |
| Rmeas | 0.151 | 0.082 | 0.847 |
| Rpim | 0.065 | 0.036 | 0.362 |
| Total number of observations | 236589 | ||
| Number of reflections | 44358 | 1446 | 6451 |
| <I/σ(I)> | 6.6 | 2 | |
| Completeness [%] | 98.6 | 98 | 98.7 |
| Redundancy | 5.3 | 5.1 | 5.3 |
| CC(1/2) | 0.996 | 0.997 | 0.693 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 20% PEG3350, 10% ethylene glycol, 0.2M sodium nitrate |
