6R5D

Crystal structure of the Pri1 subunit of human primase bound to dATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-30
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91741
Spacegroup name	C 2 2 21
Unit cell lengths	110.520, 117.290, 151.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.390 - 1.950
R-factor	0.1799
Rwork	0.178
R-free	0.21450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rb4
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260.0000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.390	2.070
High resolution limit [Å]	1.950	1.950
Number of reflections	71418	11299
<I/σ(I)>	14.56	1.33
Completeness [%]	99.2	98
Redundancy	6.75
CC(1/2)	0.999	0.746

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	23% PEG3350, 10% ethylene glycol, 200 mM Na/K tartrate