6R0Q
Mutant cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with thalidomide metabolite alpha-(o-carboxybenzamido)glutarimide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-25 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.728, 59.163, 61.751 |
| Unit cell angles | 90.00, 105.59, 90.00 |
Refinement procedure
| Resolution | 49.170 - 1.500 |
| R-factor | 0.15943 |
| Rwork | 0.158 |
| R-free | 0.19039 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v2y |
| RMSD bond length | 0.037 |
| RMSD bond angle | 4.409 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.170 | 1.590 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.080 | 0.880 |
| Number of reflections | 68423 | 10911 |
| <I/σ(I)> | 9.7 | 1.15 |
| Completeness [%] | 99.0 | 97.5 |
| Redundancy | 3.37 | 3.14 |
| CC(1/2) | 0.998 | 0.560 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 20 % PEG 3350, 0.2 M Ammonium acetate |
