6QZS
14-3-3 sigma in complex with FOXO1 pS256 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.429, 104.619, 156.866 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.800 - 1.900 |
| Rwork | 0.182 |
| R-free | 0.21580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mhr |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.800 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.105 | 0.847 |
| Rmeas | 0.113 | 0.771 |
| Number of reflections | 83148 | 4011 |
| <I/σ(I)> | 13.02 | 1.71 |
| Completeness [%] | 100.0 | 97.2 |
| Redundancy | 12.7 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277.15 | 0.1 M Bis-Tris propane, pH7 0.2 M Sodium citrate tribasic dihydrate 20 % w/v PEG 3350 10 % w/v Glycerol |
