6QMQ

NF-YB/C Heterodimer in Complex with NF-YA-derived Peptide Stabilized with C8-Hydrocarbon Linker

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-29
Detector	PSI PILATUS 6M
Wavelength(s)	0.977930
Spacegroup name	P 21 21 21
Unit cell lengths	45.010, 52.210, 72.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.890 - 2.500
R-factor	0.1795
Rwork	0.172
R-free	0.24120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4csr
RMSD bond length	0.014
RMSD bond angle	1.627
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.600
High resolution limit [Å]	2.500	2.500
Number of reflections	6325	682
<I/σ(I)>	11.07	2.18
Completeness [%]	99.6	100
Redundancy	12.2	12.9
CC(1/2)	0.997	0.665

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277.15	0.1 M sodium cacodylate pH6.5 0.2 M calcium acetate 37% PEG600