6QMQ
NF-YB/C Heterodimer in Complex with NF-YA-derived Peptide Stabilized with C8-Hydrocarbon Linker
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-29 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.977930 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.010, 52.210, 72.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.890 - 2.500 |
| R-factor | 0.1795 |
| Rwork | 0.172 |
| R-free | 0.24120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4csr |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.627 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 6325 | 682 |
| <I/σ(I)> | 11.07 | 2.18 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 12.2 | 12.9 |
| CC(1/2) | 0.997 | 0.665 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277.15 | 0.1 M sodium cacodylate pH6.5 0.2 M calcium acetate 37% PEG600 |
