6QDX

Structure of E.coli RlmJ in complex with a bisubstrate analogue (BA4)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98007
Spacegroup name	P 1 21 1
Unit cell lengths	66.420, 170.730, 66.590
Unit cell angles	90.00, 118.32, 90.00

Refinement procedure

Resolution	48.323 - 2.100
R-factor	0.221
Rwork	0.218
R-free	0.27340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4blv
RMSD bond length	0.008
RMSD bond angle	1.051
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.323	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.207	0.777
Number of reflections	75173
<I/σ(I)>	5.63
Completeness [%]	99.0
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.1 M MMT pH 6.0, 25% (w/v) PEG1500.