6QAV
Crystal structure of ULK2 in complexed with MRT68921
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-08 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.648, 69.050, 107.710 |
| Unit cell angles | 90.00, 97.93, 90.00 |
Refinement procedure
| Resolution | 57.970 - 2.050 |
| R-factor | 0.19696 |
| Rwork | 0.195 |
| R-free | 0.23085 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wno |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.267 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.970 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.078 | 0.657 |
| Number of reflections | 68365 | 6706 |
| <I/σ(I)> | 11.7 | 2.4 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 5.3 | 5.3 |
| CC(1/2) | 0.998 | 0.842 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 37.5% PEG 3350, 0.1 M sodium citrate, pH 5.9, 0.15 M MgCl2, 0.1 M bis-tris, pH 5.5, 5% glycerol |
