6QAS
Crystal structure of ULK1 in complexed with PF-03814735
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 74.241, 74.241, 222.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.470 - 1.750 |
| R-factor | 0.17146 |
| Rwork | 0.170 |
| R-free | 0.19651 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wno |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.541 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.470 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.079 | 0.777 |
| Number of reflections | 63838 | 6154 |
| <I/σ(I)> | 14.4 | 2.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.2 | 9.4 |
| CC(1/2) | 0.997 | 0.806 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293.15 | 2.8 M Ammonium sulfate, 0.1 M citrate, pH 5.6 |
