6Q20

Crystal structure of human 1E01 Fab in complex with influenza virus neuraminidase from A/Japan/305/1957 (H2N2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97946
Spacegroup name	P 4 21 2
Unit cell lengths	160.452, 160.452, 84.173
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.042 - 2.450
R-factor	0.2201
Rwork	0.217
R-free	0.27240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tia 5itb 4fql
RMSD bond length	0.003
RMSD bond angle	0.665
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.042	2.490
High resolution limit [Å]	2.450	2.450
Rpim	0.030	0.300
Number of reflections	40758	1967
<I/σ(I)>	22.5	1.1
Completeness [%]	99.5	98.2
Redundancy	13.8	10.1
CC(1/2)	0.998	0.942

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% (v/v) glycerol, 24% (w/v) PEG1500