6Q0U
Structure of the Erbin PDZ variant E-6a with a high-affinity C-terminal peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.590, 38.090, 38.450 |
| Unit cell angles | 83.04, 74.99, 63.66 |
Refinement procedure
| Resolution | 37.140 - 1.890 |
| R-factor | 0.184 |
| Rwork | 0.178 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Swiss-Modeller model of this PDZ |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.140 | 1.990 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.051 | 0.232 |
| Number of reflections | 12494 | 4051 |
| <I/σ(I)> | 10.4 | 3.4 |
| Completeness [%] | 91.1 | 73.2 |
| Redundancy | 2.4 | 2.4 |
| CC(1/2) | 0.996 | 0.941 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 14% PEG3350,200 mM MgCl2, 100 mM MES 6.0. Cryoprotected with this condition plus 25% ethylene glycol |
