6PPN

Structure of S. pombe Lsm2-8 with unprocessed U6 snRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979183
Spacegroup name	P 2 21 21
Unit cell lengths	70.280, 135.390, 168.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.700 - 1.910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4emg 4emh 4emk 4c92 4m7d
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	168.000	2.080
High resolution limit [Å]	1.900	1.900
Number of reflections	80785	4039
<I/σ(I)>	17.2
Completeness [%]	63.3
Redundancy	13.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.4	289	200 mM KCl, 50 mM sodium HEPES pH 7.4, 35 % Pentaerythritol propoxylate (5/4 PO/OH)