6PKG
Zebrafish N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) catalytic domain auto-inhibited by pro-peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-03 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.7712 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 124.356, 124.356, 278.792 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.658 - 2.800 |
R-factor | 0.184 |
Rwork | 0.183 |
R-free | 0.20810 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.542 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX ((1.15_3459: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 90810 | 4913 |
<I/σ(I)> | 16.1 | |
Completeness [%] | 98.9 | |
Redundancy | 20 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 295 | 50% PEG 200, 0.1M HEPES pH 6.4, soaked in 1M NaI |